Part:BBa_K5142036
Overlapping early/late poxviral promoter
This part includes a synthetic vaccinia virus promoter. This part can drive the transcription of its downstream element with high efficiency ONLY in poxviral factory, a specific region in cytoplasm for poxvirus replication stablished during poxvirus infection in cells, which means that this promoter cannot be used to drive transcription in nuclei or mitochondria. In this project, we used this promoter to drive the expression of the mutant A27L (A27L-3Stop).
This part was synthesized according to Modified Vaccinia Ankara virus clone VACV_MVA-HANP genomic sequence (GenBank ID: OQ818667.1, 151,027 to 151,091 bp).
The 5’-end nucleotide of the promoter and the 3’-end nucleotide of Biobrick prefix are both G, thus we delete one G at the 5’ end of the promoter to reduce the size of the part.
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 97 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 98
Illegal PstI site found at 112
Illegal NotI site found at 7
Illegal NotI site found at 105 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal BamHI site found at 83 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 98 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 98
Illegal PstI site found at 112 - 1000COMPATIBLE WITH RFC[1000]
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